Indeed, enrichment analysis highlighted a network of proteins strongly connected with the down-regulated ones in ALS (Fig. 2e), which represented some of the key members of the SNARE machinery such as SNAP91, STX12, STX1B, STCBP1, SYT1, SYP and SYN1 [61]. This evidence concerns the gene STX1B and amyotrophic lateral sclerosis.