At the beginning of this study, we researched the roles of 14 m5C regulators, including 11 writers (NSUN2-7, DNMT1, TRDMT1, DNMT3A, DNMT3B and NOP2), 2 readers (YBX1 and ALYREF) and 1 eraser (TET2), in CRC. The gene discussed is DNMT3B; the disease is colorectal carcinoma.