In this study, LPS and cecum ligation and puncture (CLP) were utilized to establish an inflammatory cell model and a murine model of sepsis-induced liver injury, respectively, to explore whether PICK1 can plays a protective role in sepsis-induced ALI by suppressing the TLR4/NF-κB pathway signaling through in vitro and in vivo experiments. This evidence concerns the gene TLR4 and Sepsis.