Additionally, the levels of IFN-γ in the Nil and TB antigen tubes of ATB patients in the training and validation cohorts were significantly higher than those of IATB patients (P < 0.001), while the levels of IFN-γ in the mitogen tube of ATB patients in the two cohorts were significantly lower than those of IATB patients (P < 0.001). The gene discussed is IFNG; the disease is tuberculosis.