MYC and neoplasm: While the glutamine concentration is limited in the tumor environment of PDAC25, which may minimize the generation of α-KG from glutamine, to ask if abnormal expression of KRAS, c-Myc, or IscU2 can also affect the level of α-KG, we then determined α-KG level in KRAS, c-Myc, or IscU2 depletion cells with limited glutamine supplementation (0.5 mM) (Supplementary Fig. S3m).