VIM and T-cell acute lymphoblastic leukemia: In Jurkat cells, which are commonly used in studies of acute T cell leukemia, T cell signaling, as well as used as an expression system for various chemokine receptors (37), we transiently transfected suspension cells and successfully tagged five out of six targeted proteins except for VIM (Fig. 2 C and D, and SI Appendix, Fig. S2 E and G).