Furthermore, to mimic microglial activation in ischemic stroke in vitro, we induced neuronal injury by incubating neurons in oxygen-glucose deprivation (OGD) conditions and then treated WT and Nr4a1-/- primary microglia with neuron-conditioned media (NCM) from control or OGD-exposed neurons (S6A Fig). This evidence concerns the gene NR4A1 and ischemic stroke.