Sequencing of this cohort contributed to first descriptions of several genes with novel or recent polymicrogyria associations (TMEM161B, SCN2A, KIF26A, QRICH1, PANX1, and MAN2C1), whether as phenotypic expansions of known neurological syndromes or as a syndrome with brain disease for the first time (PANX1). Here, TMEM161B is linked to brain disorder.