TERF1 and type 2 diabetes mellitus: The biological function of downstream target genes was also evaluated by enrichment analysis.<h4>Results</h4>qRT-PCR evaluation identified a tsRNA with up-regulated expression (tRF-1-28-Glu-TTC-3-M2) and a tsRNA with down-regulated expression (tRF-1-31-His-GTG-1), both of which may be involved in metabolic and energy-related processes.<h4>Conclusion</h4>Dysregulation of tsRNA expression in overweight patients with T2DM suggests a potential role for tsRNA in the development of T2DM.