Compared with REP TIL baseline measurements, we observed a moderate increase in IFNγ and TNFα expression in the CD3+ (median 0.34% vs. 2.27%), CD4+ (0.19% vs. 0.74%), and CD8+ (0.13% vs. 0.78%) T cells after 48 hours of co-culture (Fig. 2A), but not at 6 hours (Supplementary Fig. S7A), suggesting that REP TILs increase their tumor reactivity with prolonged exposure to the matching tumor cells. This evidence concerns the gene CD4 and neoplasm.