CD4 and neoplasm: LAG-3 expression decreased in CD4+ (13.55% vs. 1.84%, P = 0.048) and CD8+ T cells (45.55% vs. 22.94%, P = 0.029) after 6 hours of co-culture with tumor cells (Fig. 2B), but similar trends were not observed at 48 hours of co-culture (Supplementary Fig. S7D).