To further confirm the role of NKG2D activating ligands in tumor regression, we overexpressed each of these ligands by stable transfection of their cDNAs into BVE-Ctnnb1wt cells and then injected these ligand-expressing tumor cells (1 × 106) into nude mice (n=5) to observe tumor growth for 4 weeks. The gene discussed is KLRK1; the disease is neoplasm.