From fecal 16S rRNA sequencing, we first analyzed the gut microbiota profile in different groups and found that the α diversity of the gut microbiota, including the ACE and Chao1 index, was significantly reduced in patients with liver cirrhosis (LC) compared with healthy controls (HCs) (p<0.05) (Figures 1A, B). The gene discussed is ACE; the disease is laryngotracheoesophageal cleft.