To investigate the effect of human ApoE isoforms on αSynΔC positive pathology, we generated mouse models of synucleinopathy with the background of different human APOE genotypes, by backcrossing the offspring of M83+/+ mice and homozygous humanized APOE ε4 or ε3 mice with M83+/− mice for at least 2 generations to APOE ε4+/+/M83+/− and APOE ε3+/+/M83+/− mice (see Materials and Methods). Here, APOE is linked to synucleinopathy.