To determine if A1EVs can affect neuron differentiation or drive neurite growth, 1 × 108 A1EVs were added to cultured PC-12 cells, a rat pheochromocytoma line of the adrenal medulla that expresses the nerve growth factor (NGF) receptor and respond to NGF by generating plasma membrane projections that share similarities to neurites. Here, NGFR is linked to hereditary pheochromocytoma-paraganglioma.