The tumor samples were assembled into standard core-based tissue microarrays (TMAs) [9] and IF-IHC was performed for breast cancer relevant proteins including nuclear protein Ki-67 [9, 10], Programmed cell death 1 ligand 2(PD-L2) [11], and Progesterone receptor (PR) [12]. The gene discussed is PDCD1LG2; the disease is neoplasm.