Overexpression of LDHA or LDHA Y10F was validated by western blot with anti-Flag antibody as shown in Supplementary Fig. 2A. The CCK-8 assay showed that co-transfection of LDHA but not LDHA Y10F could rescue the reduced proliferation of melanoma cells by CCHE1 depletion (Supplementary Figure S2B). This evidence concerns the gene CCEPR and melanoma.