To further explore the role of MCSF in ZIC2-induced M2 phenotype polarization of TAMs, we transfected ZIC2-overexpressed tumor cells with MCSF-specific siRNA, then cocultured them with macrophages for 3–5 days or added MCSF Receptor (MCSFR) inhibitor Pexidartinib to the bottom well of transwell plates to treat the macrophages cocultured with ZIC2-overexpressed tumor cells in order to abrogate MCSF/MCSFR axis (Fig. 5B, C). This evidence concerns the gene CSF1R and neoplasm.