Under MCSF-siRNA or pexidartinib treatment, the expression of the M2-related surface marker ARG-1 was reduced (Fig. 5C), suggesting that interfering MCSF-MCSFR axis between the interactions of tumor cells and macrophages might inhibit the M2-like polarization of macrophages induced by ZIC2-overexpression tumor cells. Here, ZIC2 is linked to neoplasm.