The expression of SBEM protein in cancer cells of miR-186-5p mimic + 740Y-P group was significantly decreased than the miR-186-5p mimic NC + 740Y-P group, while the protein levels of p-PI3K, p-AKT, MMP1, MMP3 and MMP9 were not significantly changed, confirming that SBEM affects the expression of functional proteins related to cell migration, invasion and proliferation by regulating the phosphorylation of PI3K and AKT, thereby influencing the migration, invasion and proliferation of cancer cells. This evidence concerns the gene MMP9 and cancer.