The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases were adopted for the differential analysis of CDC42 expression in pan-cancer, and further glioma RNA-seq data and clinical information were downloaded from TCGA and Chinese Glioma Genome Atlas (CGGA) databases to explore the relationship between CDC42 and glioma development through a collection of bioinformatics and survival analysis. This evidence concerns the gene CDC42 and glioma.