Colony formation assay (Fig. 4C) displayed the lower number of colonies of HCC-LM3 and PLC in the RNAi groups than that in the NC group after two weeks of incubation (59 ± 9, 46 ± 12, 58 ± 8 vs. 138 ± 17 for RNAi-1, RNAi-2, RNAi-3 vs. control in HCC-LM3, P = 0.0020, 0.0014, 0.0017 respectively; 31 ± 7, 35 ± 10, 35 ± 5 vs. 126 ± 20 for RNAi-1, RNAi-2, RNAi-3 vs. control in PLC, P = 0.0014, 0.0019, 0.0014 respectively). The gene discussed is HSPG2; the disease is hepatocellular carcinoma.