Using the CRC TMA (n = 787) to stain for multiplex immunohistochemistry, the panel of immune cells; T lymphocyte (CD3, FOXP3), macrophages (CD68, CD163) and granulocyte (CD66b) were stained together with αSMA and PanCK for tissue segmentation (Figure 5). The gene discussed is ACTA1; the disease is colorectal carcinoma.