A single band was observed by western blot using a commercial 293T cell lysate overexpressed with S100A2 and a faint band was observed for HCT116 cell line lysate, known to express S100A2 at low levels, and a stronger band was shown for MDA-MB-231 breast cancer cell line lysate, known to express S100A2 at higher levels. Here, S100A2 is linked to breast cancer.