We identified “stem-like” cell populations (CD44+ and CD44+/CD133+) in aggressive metastatic prostate cancer subtypes, which was confirmed by the RNA-seq and subclonal overexpression of several EMT markers (VIM, HAS3, S100A6, ANXA2P2, ANXA2, ANXA3, AHNAK2, LOXL2, TGFB, TGFBR2, UCHL1, CD44, CD55, and CD109). Here, CD109 is linked to Familial prostate cancer.