The CD8+ and IFN‐γ+ infiltrating lymphocytes within the local tumor microenvironment were identified via immunofluorescence analysis using anti‐CD8 (green) and anti‐IFN‐γ Ab (red) to ascertain whether the hsCD80‐transformed L. lactis promotes T lymphocyte activation and antitumor immunity. This evidence concerns the gene CD8A and neoplasm.