INSR and reading: TIMER database was then used to analyze the correlation of 6 DEPs and 5 key mutant gene with the extent of immune infiltration, the results showed that FOLR2, INSR, CCND1, KRAS and PTEN, highly expressed in the microenvironment in colon adenocarcinoma (COAD) and rectum adenocarcinoma (READ), were negatively correlated with tumor purity, and was positively correlated with immune infiltration (Figure 6C).