EHD1 and neoplasm: To assess if the marked reduction in pro-oncogenic traits seen in vitro translates into impaired tumorigenesis in vivo, we implanted TC71 NTC, EHD1-KO, and mEHD1 rescue cell lines engineered with a lentiviral mCherry-enhanced luciferase reporter46 in the tibias of Nude mice (n = 8 per group at the beginning) and monitored tumor growth by bioluminescence imaging.