PTK7 and hepatocellular carcinoma: Specifically, we chose Jurkat cells, an ALL lymphocytecell line, and HepG2 cells, a human hepatoma cell line, as both havebeen shown to have increased and similar PTK7 levels38 that have been correlated to cell proliferation and metastasis.39,40 Both cell lines were incubated with increasing concentrations ofsgc8c(27)-1 followed by labeling with a streptavidin–phycoerythrin(SA–PE) construct for 5 min at room temperature.