Six weeks after instillation of Adeno-Cas9-Cre-Trim24/Ret gRNA virus in C57BL/6-TP53fl/fl mice, multiple tumor foci were observed in the fixed lung lobes (Fig. 3A), demonstrating that the Trim24 and Ret sgRNAs induced gene rearrangement-dependent tumorigenesis as previously observed with Eml4-Alk rearrangements (Kleczko et al., 2023a; Maddalo et al., 2014). This evidence concerns the gene ALK and neoplasm.