To exclude any possibility of transgene expression in the non-hematopoietic compartment we first transplanted 2 × 106 BM cells, isolated from either SCLtTA/BCR::ABL1 CML or SCLtTA control mice into 10 Gy irradiated recipients and allowed these cells to engraft for 1 week before inducing BCR::ABL1 expression. The gene discussed is ABL1; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.