For exploring NDUFS1’s function using gain-of-function strategy, the overexpression of NDUFS1 were used to investigate its role in mediating the radiosensitization of colorectal cancer (Shi et al., 2021) or in miR-3130-5p mediated invasiveness of lung adenocarcinoma (Zhan et al., 2021). This evidence concerns the gene NDUFS1 and lung adenocarcinoma.