Because the GSEA indicated that the expression levels of IFN-α and-γ response gene sets were the lowest in H445Y compared with S450L, and because elevated expression levels of IFN response genes are associated with active TB disease (Singhania et al., 2018), six IFN-responsive genes, BATF2, SERPING1, UBE2L6, VAMP5, IFI44, and STAT1 in the hallmark gene sets (Supplementary Table S10), reported to be associated with TB (Gong et al., 2021), were chosen for validation using qRT-PCR of individual blood samples. The gene discussed is VAMP5; the disease is tuberculosis.