It was reported that mitochondrial DNA (mtDNA) in the cytosol or extracellular milieu can drive IFN‐I production to promote human lupus.[24] Therefore, we treated WT and Ebi2‐cKO PEMs with the BCL‐XL and BCL‐2 inhibitor ABT‐737 plus the pan‐caspase inhibitor QVD‐OPh[25] to release mtDNA into the cytoplasm, which increased the transcription of Ifnb, and ISGs including the chemokines Cxcl10 and Ccl2, the GTP‐binding protein Mx2, the IFN‐induced protein with tetratricopeptide repeats 2 (Ifit2), Irf7, Ifna4, and Ifna in Ebi2‐cKO PEMs (Figure 4A and Figure S4A, Supporting Information). The gene discussed is IFNB1; the disease is systemic lupus erythematosus.