To investigate whether additional glutamate from GBM-microglia assembloid released by either microglia or GBM cells, we neutralized cytokines in the GBM conditional medium using neutralizing antibodies targeting GM-CSF (aGM-CSF), IL-6 (aIL-6), and G-CSF (aG-CSF) and treated the neutralized conditional medium on single-culture microglia. Here, CSF3 is linked to glioblastoma.