Finally, we next tested whether EA administration exerted anti-anxiety effects by regulating the activity of GABAergic neurons or GABABRs. For this purpose, we also performed OFT and EZM experiments on rats with the combined treatment of EA and AAV-VGAT-hM3D-mCherry virus or baclofen (Fig. 5D, E). The gene discussed is SLC32A1; the disease is Anxiety.