Importantly, there was no significant correlation between RNF8 mRNA levels and the protein levels of DDR markers in the TCGA RPPA dataset, including pCHK2_pT68, pCHK1_pS296, pCHK1_pS345, 53BP1 and MRE11, suggesting that RNF8’s prognostic value in glioma may not be related to its role in DDR (Fig. S5A). Here, MRE11 is linked to central nervous system cancer.