For example, SW480 (Apc 1338/-), DLD1 (Apc 1417/-), and HT29 (Apc 853/1555) CRC cells, expressing different truncated forms of APC49, displayed increased free cholesterol compared to WT APC-expressing HCT116 (Apc +/+ βcat −/+) and HCT116Δ (Apc +/+ βcat Δ/+) CRC cells (Fig. 2C). The gene discussed is APC; the disease is colorectal carcinoma.