LGR5 and colorectal carcinoma: Interestingly, similar to free cholesterol data from mice colonic crypts and Lgr5+ stem cells as well as human CRC cultured colonocytes and PDOs (Figs. 2 and 3), oncogenic APC-induced a gene dose effect on plasma membrane order (double mutant allele Apc 580/580 > single allele Apc 580/+ > WT Apc +/+) (Fig. 5B, C).