To start with, we investigated the expression profile of SLC35A2 gene in pan-cancer and its association with clinical outcomes via TIMER, GEPIA, UALCAN and Kaplan–Meier Plotter, which consistently indicated the altered SLC35A2 expression in various cancer tissues, that were BLCA, BRCA, CHOL, COAD, ESCA, HNSC, LIHC, LUAD, LUSC, PRAD, READ, UCEC. This evidence concerns the gene SLC35A2 and cancer.