Here, we show the particular combination of paper-microfluidictechnology, electrochemical transduction, and magnetic nanoparticle-basedimmunoassay approaches to produce a unique, compact, and easily deployablemultiplex device to simultaneously measure interleukin-8, tumor necrosisfactor-α, and myeloperoxidase biomarkers in sputum, developedwith the aim of facilitating the timely detection of acute exacerbationsof chronic obstructive pulmonary disease. Here, MPO is linked to chronic obstructive pulmonary disease.