<h4>Purpose</h4>To investigate the effectiveness of an automatic analysis framework based on 3D-CT multi-scale features in predicting Ki67 expression levels in substantial renal cell carcinoma (RCC).<h4>Methods</h4>This retrospective study was conducted using multi-center cohorts consisting of 588 participants with pathologically confirmed RCC. The gene discussed is MKI67; the disease is hereditary clear cell renal cell carcinoma.