As a result, we figured out that m6A regulatory factors might be a prospective target for CSCs in HCC, then by analyzing the scRNA-seq data of HCC and followed by RT-qPCR validation, we found that the ten hub m6A regulatory factors were positively related to the CSCs markers CD24, EPCAM, and CD133 and highly expressed in LCSCs. This evidence concerns the gene CD24 and hepatocellular carcinoma.