In our study, we discovered that decreased expression of Wnt, β-catenin, and c-Myc and enhanced expression of phosphorylated Gsk-3β were observed in NB cells after circ_0000285 knockdown, suggesting that circ_0000285 knockdown inactivated the Wnt/β-catenin signaling pathway. The gene discussed is GSK3B; the disease is neuroblastoma.