PMEL and melanoma: In order to assess if the combination of restored MDA expression and increased surface HLA-ABC upon RSK inhibition translates into an improved recognition by and activation of the immune system, primary human cytotoxic T cells were transduced with HLA-A*02-restricted, MDA-specific TCRs (gp100-TCR, Melan-A-TCR) and co-cultured with HLA-A*02-positive melanoma cell lines (Fig. 5C).