PLIN3 and hepatocellular carcinoma: The mRNA and protein levels of PLIN3 in liver tissues, including normal tissue, NAFL, HCC without or with NAFL, were examined by qRT-PCR (Fig. 5C), immunohistochemistry (Fig. 5D), and western blot (Fig. 5E), which indicated that LDs accumulation in the liver could promote the transcription and protein expression of PLIN3.