Equivalent uptake of WT and replication-incompetent (ΔsifA) bacteria was observed after 2 h of infection in rufy3-/- and WT RAW cells, showing that iRUFY3 is not involved with bacterial phagocytosis (Fig. 7b), although It is found in the vicinity of some SCVs, together with LAMP1 and the bacterial effector PipB245 at 16 h post-infection (Fig. 7c and 7d). Here, LAMP1 is linked to infection.