Given the significant reduction in Ca2+ burden and dendritic glutamatergic excitotoxicity observed in the Trpc3KO Purkinje neurons in vitro, the contribution of expression of Trpc3 and Trpc1, Trpc3, Trpc6, and Trpc7 genes in combination (in a Trpc1/3/6/7 quadruple knockout (TrpcQKO)) was assessed in vivo using the photothrombotic model of focal ischemia. The gene discussed is TRPC1; the disease is ischemia.