To validate the role of HGF in 4T1 BC progression in bone and metastasis to lung in the 4T1 BC mice, we determined the effects of crizotinib, a small compound of c-Met/ALK tyrosine kinase inhibitor, which has been shown to have beneficial effects in patients with non-small cell lung cancer 43 and breast cancer 44. This evidence concerns the gene HGF and breast cancer.