Finally, we examined the levels of PRMT5, KLF5, cyclin D1, slug, p‐Akt and p‐GSK3β expression in the primary tumour samples and observed that inhibition or downregulation of PRMT5 led to a reduction in the protein expression of these markers that were closely related to the epithelial–mesenchymal transition (EMT) and metastasis (Figure 6D,H). The gene discussed is CCND1; the disease is neoplasm.