AGER and breast cancer: Then, we stimulated both MCF-7 and ZR75 BC cells with Ins, alone and in combination with increasing concentrations of the small molecule RAGE inhibitor FPS-ZM1 (2 μM ÷ 10 μM) (concentrations that did not interfere with cell viability as shown in Additional file S2: Fig. S2 K-L) [24], to evaluate whether the inhibition of RAGE signaling may affect Ins-induced stimulatory responses.