In this retrospective study, we included multiple potentially influencing factors for HBL and found by multiple linear regression analysis that BMI, surgical duration, and Hb loss were positively associated with HBL, while preoperative Hb was negatively associated with HBL and the association was statistically significant, whereas age, Alb loss, gender, ASA class, major preoperative diagnosis, and hypertension did not seem to be an independent risk factor for HBL. Here, GSTM1 is linked to Hypertension.