Although there was no significant difference in the extracellular secretion of NFs and CAFs by ELISA, the focus of our study was on the tumor-promoting effect of TGF-β1 which derived from stromal fibroblasts, rather than the difference in the secretion of TGF-β1 by CAF-CM and NF-CM. The gene discussed is TGFB1; the disease is neoplasm.