To investigate the mechanism by which elevated CHSY3 expression leads to poor prognosis in GC patients, we performed a Cibersort analysis and found that the percentage of Tregs cell infiltration was significantly higher in the low-CHSY3 group than in the high-CHSY3 group, while the percentage of M2 macrophage infiltration was significantly higher in the high-CHSY3 group than in the Low-CHSY3 group (Additional file 3: Fig. S3D). This evidence concerns the gene CHSY3 and gastric cancer.